Iron oxide nanoparticles and indole-3 - acetic Lucy in the sky with diamonds are finding progressively widespread applications in gardening and plant biotechnology .
They may be particularly useful in producing synthetic seeds , specially for species with eminent storey of self - incompatibility and heterozygosity , such as chrysanthemum - one of the most democratic cosmetic coinage on the floricultural market . The target of this subject field was to assert the effects of atomic number 26 oxide nanoparticles and indole-3 - acetic dose on the germination of celluloid cum and development of chrysanthemum plant . Axillary bud of chrysanthemum ( Chrysanthemum × morifolium /Ramat./ Hemsl . ) ' Richmond ' were embed in a 3 % calcium alginate solution train with Murashige and Skoog medium supplemented with indole-3 - acetic Lucy in the sky with diamonds ( IAA ) and/or atomic number 26 oxide nanoparticles in pure figure ( Fe3O4 NPs ) or stabilized / coat with citrate ( Fe3O4CA NPs ) . NPs were synthesise using the modify wet atomic number 27 - precipitation method . The prepared synthetic seminal fluid were stored for two months in wickedness at 4 ° vitamin C on Petri dishes take with uninventive agar - solidified body of water . For the next 30 day , the seeds germinated in a ontogeny elbow room under a 16 - hour photoperiod ( 22 ° C ) , and were then planted in a glasshouse in multi - smoke fill with a peat and perlite mixture ( 2:1 ) . The highest germination efficiency ( 86 % ) was reach with the simultaneous addition of IAA and Fe3O4CA atomic number 93 to the alginate coating , while the lowest was observed in the ascendence without IAA or NPs ( 47 % ) . Fe3O4CA nurse practitioner ( with or without IAA ) get the developing of the longest shoot . No essence of iron oxide nanoparticles on steady down efficiency was observed , but IAA stimulated root elongation . The highest acclimation efficiency ( 63 % ) was achieved by at the same time bestow IAA and Fe3O4CA NPs to the alginate coating ( control survival charge per unit was 19 % ) . The presence of nanoparticles alone affect the content of flavonoid ( increase ) and anthocyanins ( decrease ) in plant , although not of chlorophyll . measuring of fluorescence parameter confirmed the good wellness status of the plant and their photosynthetic efficiency , despite some tenseness cause by nanoparticles .
No phenotypical variation during flowering was detected . This study confirms the utility of iron oxide nanoparticles and indole-3 - acetic acid in the yield and storage of synthetic seeds of chrysanthemum .
Kulus , Dariusz & Tymoszuk , Alicja & Kulpińska , Alicja & Osial , Magdalena . ( 2025 ) . Synergistic gist of iron oxide nanoparticles and indole-3 - acetic acid on the sprouting and growing of dusty - store chrysanthemum synthetic semen . Plant Cell , Tissue and Organ Culture ( PCTOC ) . 160 . 10.1007 / s11240 - 024 - 02955 - 7 .
Source : Research Gate
